用双层
PC-3前列腺癌细胞将两半固定在一起，形成双悬臂梁(DCB)
标本。将试件固定在底角处，对同一端的
上角进行位移，记录力-位移曲线
。这个上角被移开，直到裂纹(
试样中细胞生长被选择性抑制的部分)通过细胞层扩展
。裂纹
穿过单元层的临界值与初始
裂纹长度结合，通过柔度-能量法确定韧性(Ripling, et al. 1971)。对
试样进行有限元分析的方法越来越复杂。基本模型涉及在一个有限元模型上计算j积分(Rice 1968)，该模型具有
预测的施加于其上的力的临界值。第一个扩展涉及
将细胞层建模为弹簧，应用预定的临界力，
并计算j积分(Song 1994)。最后的增强
涉及通过在F.E.模型中使用
内聚元素对细胞使用牵引分离定律。 The traction-separation law is determined by
experimental work. This model is then used to determine the critical value of
force and toughness via the J-integral.

Rice, J. R. (1968). "A path independent integral and the approximate
analysis of strain concentration by notches and cracks." ASME Journal
of Applied Mechanics 35: 379-386.

Ripling, E. J., S. Mostovoy, et al. (1971). Fracture Mechanics: A Tool for Evaluating Structural Adhesives, Taylor & Francis. 3: 107 - 123.

Song, S. J., Waas, A. M. (1994). " A spring foundation model for mode I failure of laminated composites based on an energy criterion." J. Eng. Mater. Technol. (Trans. ASME) 116(4): 512-516.

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